I am looking for a simple and low-cost method of monitoring the cholesterol content in the cell membranes. My initial idea was to stain them with cholera toxin (anti-GM1 ganglioside) to get information about lipid rafts, so indirectly about the cholesterol content.
Do you have any better ideas?
Dear Wojciech,
You could also consider cholesterol quantitating kits (e.g. https://www.sigmaaldrich.com/catalog/product/sigma/mak043).
Besides membrane fluidity mentioned in the previous post the membrane dipole potential is also correlated with cholesterol, although obviously there are factors other than cholesterol which influence the dipole potential, But this statement also holds for fluidity.
Another possibility is anti-cholesterol antibodies.
The attached paper is about the dipole potential and anti-cholesterol antibodies.
All the best,
Peter
Article The dipole potential correlates with lipid raft markers in t...
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