Literature on snowflake aggregation?

I am looking for literature suggestions on the topic of snow aggregation. My main interest is on the aggregation process itself (rather than papers describing aggregate snowflakes characteristics)...

19 February 2021 2,197 3 View

ASTM or ISO standard test for duplex coatings?

Dear all, I would like to conduct scratch and linear reciprocating tribo-test on my magensium alloy, which has a plasma electrolytic oxide (PEO) layer of about 20 um and 10 - 20 um of Parylene...

25 January 2021 9,709 2 View

Does anyone know about a good article/book about the correlation between the water crisis and the macroeconomic decisions?

I'm writing a thesis about the macroeconomic issues to face during a water crisis

25 December 2020 6,321 1 View

Any data or studies available on wall thermal insulation with glass bottles?

I am searching for data and studies about thermal performances of walls insulated using recycled unbroken glass bottles, to be used as part of the structure itself, or as filling material of a...

29 November 2020 7,792 2 View

EDX sample preparation of a coating/metal cross section?

The sample is piece a magnesium alloy (dimension: length - 20mm, width - 9.7mm, thickness - 3.5mm), which is coated with epoxy and silicone coating on each side of the surface. The coatings do not...

05 January 2020 3,375 3 View

Cloudy debris in the middle of a flask??

Hi everybody, I've started to grow up bacteria in liquid medium in order to set up a growth curve but everytime I try to start it there is this cloud in the middle of the flask. The bacteria...

26 March 2018 4,656 2 View

What are the basics to maintain a micropipette in good working conditions?

26 November 2014 9,393 4 View

Does anybody know the difference between GISTIC 2.0 and ABSOLUTE algorithms, if there is any?

While reading the literature,it is not very clear to me the rationale of using one or the another.

22 June 2014 4,609 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible to do piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch?

How to do Piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch? Piezoelectric coupling between the rectangular substrate and rectangular...

02 March 2021 9,833 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View