How to modify a plasmid DNA with a thiol group or any functional groups for coupling?

04 August 2019 1 319 Report

I am trying to conjugate a plasmid DNA with an -SH activated PEG. The thiol group modification is possible for linear single strand DNA. I wonder if it possible to introduce in a plasmid DNA, too.

Eimantas Astromskas

Hi, You should look at Klimasauskas work for this. Start with PMID: 23042683 and PMID: 28111014 .

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