How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How the wettability of rock sample can be changed by ageing in pure hydrocarbon oils only?

I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...

27 February 2021 5,383 3 View

Gromacs force field and water model recommended for protein hydrophobic study?

Dear All, I received a comment from reviewer about asking me selection of force field and water model. I choose OPLS-AAL and 3-point water model. They mentioned the combination of OPLS-AAL and SPC...

13 February 2021 1,364 3 View

Is it possible to find out the wettability and contact angle of water on the rough or corrugated substrate?

First of all, whether it is possible to identify the presence of water and its amount on the Silicon substrate by using only FT-IR spectroscopy. Can ATR-FTIR spectroscopy find out the wettability...

03 February 2021 9,085 4 View

What could be possible reason of higher Drug Concentration of Liposomal formulation stability samples over time?

Hello Everyone! I have prepared liposomal formulation using Rotary Evaporator method followed by dialyzed the liposomal Suspension in Phosphate buffer to remove the un-encapsulated drug. After...

25 January 2021 7,752 4 View

Why polyethersulfone membrane is hydrophilic?

Polyethersulfone membrane are generally hydrophilic. How can I explain this in terms of chemistry, molecular structure and functional group?

03 January 2021 6,321 7 View

I am looking for polymers/biopolymers with high hydrophobicity and high gas permeability. Can any one recommend any types of polymers/biopolymers?

I am looking for polymers/biopolymers with high hydrophobicity and high gas permeability. Can any one recommend any types of polymers/biopolymers?

25 November 2020 5,887 3 View

How to remove stickiness of Sylgard?

Hello, everybody I have tried to obtain a hydrophobic surface by Sylgard-184 (PDMS). I dissolved PDMS in n-hexane and applied it to the glass substrate, then dried at 70C over night. Finally,...

17 November 2020 7,984 3 View

Hydrophobic treatment of hydrophilic spiral-shape tubing?

I was wondering if there is a simple and easy way to treat the tubing surface to make it highly hydrophobic. Here is what I am trying to do: the spiral-shape tubing will be filled with...

09 November 2020 153 3 View

Should these surfaces be hydrophobic?

I am trying to coat PDMS and Polyimide with (3-mercaptopropyl)trimethoxysilane and (3-glycidyloxypropyl)trimethoxysilane respectively. I was wondering whether these surfaces should exhibit a...

29 October 2020 5,970 4 View

How to cut the thrombin cleavage site if it is hindered by hydrophobic region?

I'm currently doing protein purification. my protein size is around 42kDa, however, my protein is attached with fusion tag which are Trx-tag and His-tag at the N terminal. I did optimize my buffer...

12 October 2020 2,083 3 View