How to merge my account with my maiden name?

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How to adjust an electrostatic potential surface in pyMol after adding phosphorylation sites?

I used I-TASSER to predict the 3D structure of my protein of interest and PyMOL with APBS plugin to add a surface showing the electrostatic potential. Then, with PyTMs plugin, I added three...

25 January 2024 6,228 0 View

Hi Researchers, could you recommend an antibody to the GIRK4 protein encoded by the Kcnj5 gene?

I am searching for antibody to rat brain tissue. I would prefer to purchase antibody for both Western blot and Immunohistochemistry. Thank you in advance.

01 January 2024 6,335 1 View

How to optimize a protein structure in PyMol after adding phospho residues?

I am using PyMol with PyTMs plugin to visualize possible phosphorylation sites on a protein. I am wondering how I can use the program to calculate and show an optimized protein structure to avoid...

26 November 2023 7,663 1 View

Does anyone had problem with Promega Annexin V, where it shows that living cells are dead and vice versa?

Hello! I have used Promega RealTime-Glo Annexin V Apoptosis Assay but the results looks very weird and I would like to know if someone else came across that kind of trouble. The highest...

15 October 2023 4,813 0 View

Is there an antigen retrieval method that doesn't involve high temperatures?

I am doing immunofluorescence on tissue sections. After the deparaffinization I do the antigen retrieval, but I think is destroying some nucleolus structures I am trying to label. Any...

22 August 2023 4,503 2 View

Does anybody know a good measure of cultural competence for children (7-9-year-old)?

I am looking for a measure/scale suitable for assessment in classrooms to measure cultural competences (cultural sensitivity and self-awareness)? I will be grateful for any advice :) Best...

02 January 2023 1,268 2 View

Additional bands on Western blot after protein overexpression? What are the additional products?

Dear Researchers, I cloned the IRF9 coding sequence into plasmid LV073. I used the Gibson assebly (NEBuilder) protocol. I cut the vector in MCS with the enzyme ScaI (blunt ends), amplified my...

26 November 2020 5,242 4 View

Has anyone pooled libraries from ChIP and RNA experiments in one lane for sequencing?

I have one lane and samples from two experiments (RNA and ChIP). Is there an option to sequence them together? I am afraid that RNA samples will "eat" all the reads. Thank you for any suggestion!

21 July 2020 2,043 4 View

What features (thickness?) does copper coating have to have to proprly prevent the carburizing?

Im planning to do the carburizing of my steel sample, but I want to prevent one part of it by copper plating and I was wandering if there is some indication about the copper coating created to be...

08 February 2020 4,958 0 View

What is the best instruments/equipment for measuring PM concentrations in outdoor air?

I am looking for an accurate portable equipment to mesure PM in the ambient air.

08 January 2020 5,194 0 View

How to generate a citation of my paper from ResearchGate?

How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...

08 August 2024 6,690 3 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

How do you delete a duplicate pdf for the same paper on ResearchGate?

The first pdf file I uploaded had an error. So I uploaded an updated, corrected pdf of that paper with a different pdf name. I dpon't want the old copy to be download or read.

07 August 2024 9,508 1 View

How to get links for copyrights for papers?

how to get links for copyrights for papers?

06 August 2024 7,410 1 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

What is the Name of my Mother in law?

Chimnya

05 August 2024 6,947 1 View

How do i get an account to upload my published papers?

need to open an account to upload my published papers

01 August 2024 9,255 1 View

About impact factor?

I have been publishing the Nepalese Journal of Agricultural Sciences (online ISSN 2091-0428; print ISSN 2091-042X) on www.nepjas.com and research gate. I was wondering how I can get an impact...

01 August 2024 1,277 1 View

What do you consider to be the most relevant elements of EEG for studying cognitive biases?

I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...

31 July 2024 7,259 1 View

Any tips on how to increase the chances of getting a response?

I've been using ResearchGate for a while now and have found many useful research papers that are beneficial for my work. However, I've noticed that many authors do not respond to my messages....

31 July 2024 7,771 5 View