Hello everyone, I need some help with the Nanodrop 2000c for dosing RNA concentration of my samples. It's the first experience for me with the nanodrop when it comes for RNA measurement, so I extract my viral RNA with QIAGEN QIamp Viral RNA mini Kit, and after the extraction, I get good concentration and good ratio on 260/280 but a very poor ratio on 260/230 even a negative ratio. So I don't know if this information is reliable or do I need to improve my extraction procedure ? and how ? and what if the measurement will be affected if the sample was in -20°C for a long time ?
Also, is it possible to measure my cDNA and do I need to select Single or Double Strand-DNA on the software ?
Thank you very much.
Hello Anass
Nanodrop comes with standard RNA solution and the machine has got high sensitivity which you can choose and whether its in micrograms or ng, its your choice but would advice you to select ug/ml. Then it will guide you to insert the standard being diluted according to the protocol and later it will ask you to insert the sample which is your RNA. sometime it is possible that your RNA is too concentrated which you would need to dilute it before taking measurements.
with cDNA, you'd need either the ChemDoc system for the expression levels or the RT-PCR.
all the best.
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