Does anyone know the recommended values (min and max) for phytic acid in ruminants?
Despite satisfactory qPCR curves for the positive control and calibrator, I observe a lack of signal from the VIC dye (reference gene) in my experimental samples after bisulfite conversion. I have...
13 May 2024 8,026 2 View
Dear all, I'm trying to amplify a genomic mRNA of a human gene for Sanger sequencing downstream application. The length of the PCR product is 500pb. The mRNA are extracted from whole blood...
11 October 2018 5,080 5 View
We are working with E.coli K12 strain and want to knock out a certain gene in its genome. We already have some concepts of sgRNA, but we do not know in which plasmid (either pCas9 or pGRB or...
09 September 2018 7,773 0 View
What is the advantages of ordinary least squares regression analysis compared to SEM ?
30 July 2018 2,069 5 View
Hello, I want to extract mRNAs from human whole blood samples for qRT-PCR and sequencing purposes. But, I don't know which method or kit reagent is the best with a higher mRNA final concentration....
19 February 2018 8,701 4 View
Hi everyone, I would like to have a value of a constant Ea, the activation energy of supercooled liquid nucleus interface of ''POM'', according to Hoffman-Lauritzen giving in the attached...
15 February 2018 8,464 0 View
I am trying to search for a better way to purify my histidine tagged protein by looking for a replacement for EDTA, since it trips the metal ions in de IMAC columns. I first used the Nikkel column...
26 January 2018 1,968 10 View
Hi community, I need to modify my thr heat equation in comsol and the variable T depending just on y. So, what is the best way to follow? Should I modify the equations from the "equation view...
28 October 2017 4,314 5 View
I want to calculate the fault cost in a medium voltage networks.
14 March 2016 6,974 3 View
is it possible that the Carbon (CH) of the terminal alkyne does not appear in the NMR DEPT 135), it behaves like a quaternary carbon ?
03 October 2015 2,980 1 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,622 4 View
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,185 6 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,418 1 View
I am working on natural remedies for de-worming in small ruminant livestocks. Part of my project involves formulation of drench-type product, which I have not much experience in. My experience is...
30 July 2024 9,806 1 View
I would like to know the role of the TFA (trifluoroacetic acid) used on reverse phase chromatography c-18 column. It's is often used with a concentration of 0.1% on the mobile phase.
23 July 2024 8,073 3 View
As mentioned in literature the addition of CaCO3 to MRS Agar media is considered as an indicator for lactic acid producing bacteria, so I wonder if we can add CaCO3 calcium carbonate to any other...
20 July 2024 7,504 0 View
I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...
19 July 2024 8,522 0 View
I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria
15 July 2024 8,890 2 View
15 July 2024 2,414 1 View