Dear Science Crowd,
I have a problem: I'd like to test if ultrasonic-treatment genreates ROS in cell culture media supplemented with FCS or BSA before this is applied to the cells. However, I mostly find tests for ROS that require living cells in the set-up. Does anyone of you have a suggestion?Thanks in advance!
OxyBURST® Green Reagents of THERMO by spectrofuorometer based
This link will help you out
https://www.thermofisher.com/content/dam/LifeTech/global/technical-reference-library/Molecular%20Probes%20Handbook/chapter-pdfs/Ch-18-Reactive-Oxygen-Species.pdf?icid=WE216841
There are options. You want to avoid dyes that require esterase cleavage (dyes with AM esters or diacetates). With all due respect to Jayant, you will want to avoid Oxyburst (D2935) for this reason (unless you first cleave the AM ester chemically).
However, you can try using dihydroethidium (DHE), CellROX Orange, or CellROX Deep Red (but not CellROX Green unless you add DNA to the solution). None of those require esterase cleavage.
The paper that Stephen recommended looks good, but be aware it requires a luminescence readout with a luminometer, which can be more sensitive, but also very short-lived signal.
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