How to measure phytase activity??

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Binder for ZnO nanoparticles in graphite paste electrode?

I have been using paraffin, but the deposited ZnO still detach from electrode. What is the best binder to modify graphite paste electrode with ZnO nanoparticles?

03 August 2024 4,624 3 View

If the emission of all greenhouse gases is stopped now, will the temperature of the earth continue to rise?

Here's a serious question: If we were to stop emitting all greenhouse gases right now, would the Earth's temperature start to cool down, or would the existing greenhouse gases continue to warm the...

30 July 2024 5,123 4 View

Wrong out put for gmx x2top?

Dear users, I would like to simulate a zeolite structure in gromacs. I got the .cif file from IZA expanded in one direction in Materials Studio and exported a .pdb file. However, when I want to...

24 July 2024 367 4 View

Is there a good macro photography kit for on-site metallurgical investigation?

I am looking to purchase a photography kit for onsite metallurgical investigations, such as capturing photos of worn areas, hot tears, cold tears etc. Could anyone suggest me a good...

22 July 2024 3,490 2 View

The measurement of cellular uptake of nanoparticles?

What protocol can be used to measure the cellular uptake of a nanoparticle? Is there a way to get more of a non-targeted nanoparticle into the cell? Why a fluorescent nanoparticle does not enter...

18 July 2024 7,153 2 View

How to define a zeolite structure with rigid framwork?

Dear Gromacs users, I would like to simulate a zeolite-water system. In different literature, it is suggested to consider a rigid framework of zeolite to decrease the computational cost. I would...

15 July 2024 4,590 1 View

How to classify 3D Bioprinting as a part of Additive Manufacturing?

Hello, I have a problem in classification of "Additive Manufacturing" techniques. There are several classification for Additive Manufacturing e.g. Powder, Extrusion, Resin etc. Actually, I am OK...

15 July 2024 139 3 View

Is there a difference between the curriculum and the style of the study?

A question about scientific methodology

13 July 2024 2,160 8 View

How to calculate Cohen's d from CI 95 and t value from a paired sample t test?

We have conducted a systematic review to investigate the effectiveness of a treatment for a psychological disorder. We aim to report effect sizes and p values of the reviewed studies but one study...

10 July 2024 7,186 4 View

Authorship for data analysis?

I participated in 2 research projects in which I was responsible for part of data analysis and figures production. Also, I spent hours trying a new statistical approach -for me- to implement in...

10 July 2024 1,226 5 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Does anyone notice the tumor regression impact of Hepa1-6 cells in HCC orthotopic mice model?

To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...

18 July 2024 9,177 2 View

What do I do with dried urine samples?

I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...

14 July 2024 6,724 0 View

How long can I store mouse tissues in neutral buffered formalin for, and how often should I add fresh formalin?

My study uses C57BL/6J mice and I am required to store portions of the important organs in neutral buffered formalin (NBF). Could I potentially keep those tissues in the formalin for a few weeks...

06 July 2024 8,360 2 View

Is still the cryptococcus sp. considered as the top most critical priority pathogen?

In the year 2022 the WHO released a fungal priority pathogen list in which Cryptococcus neoformans being placed at the top most of critical priority pathogen. Is it still a threat to human...

10 June 2024 4,055 3 View

Apart from basic bone remodeling markers, which other blood biomarkers do you think have potential to predict fractures in older adults?

Currently, there are numerous blood biomarkers that have been linked to fractures and increased fracture risk (for example, IL-6). Considering the current evidence, which ones do you think are the...

10 May 2024 6,097 2 View

Why are the mice in my control group mildly hyperglycaemic?

I am currently working towards developing a model for type 2 diabetes, using fructose and streptozotocin (STZ). Prior to injecting my C57BL/6 mice with STZ, we tested blood glucose with a...

08 May 2024 3,947 2 View

How to perform DNA extraction for Long read sequencing using QIAgen genomic buffer set kit from Aspergillus isolates?

Greetings everyone, I am currently tasked with conducting DNA extraction from Aspergillus isolates, utilizing the QIAgen genomic buffer set kit. Regrettably, not all the components outlined in the...

03 May 2024 4,985 0 View

What is the good dilution factor for mouse serum in ELISA?

I am doing IL6 ELISA to measure mouse serum IL6, but my ODs are so low even for mouse on two weeks high fat diet. My standard curve is good enough as a positive control.

28 April 2024 8,922 2 View

BLAST sequence in plus/minus and plus/plus in reverse sequence when CRISPR is performed. Are they mutants?

We are performing CRISPR on a gene from a strain of Aspergillus niger and then sequencing the results by Sanger. When we compare the gene sequence with the resulting sequence we observe in the...

25 April 2024 6,159 0 View