How to measure IFN -gamma and IL-10 in the splenocyte supernatants by ELISA after L.donovani challenge in mice?

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Wijitapure Wimalaratana

This is an unknown area to me!

Rafael Freitas

Well, you have to collect the spleen, process it to release the cells and culture them either in the presence of the antigen or with no antigen at all. After some days you collect the supernatant and measure the cytokines you want.