I wanted to test weather a test compound is inhibiting activity of INHA protein of M. tuberculosis or not.please suggest, how i can design the experiment.
dr nisha
http://www.ncbi.nlm.nih.gov/pubmed/20200152
read the full article .this might help you.
In the simulation tutorial of the aeroacoustics around a cylinder by ANSYS, recievers are placed at a location where mesh isn't present, how does the solver predict the acoustics at that location...
16 October 2020 3,368 5 View
Hi, I have a problem with cryosections while staining. Positively charged slides are used for cryosections, in spite of that during the staining process, the sections are washed away during the...
10 September 2020 3,767 3 View
I was trying to find out whether my bacteria can degrade crude oil or not using DCPIP method that changes color after oxidizing hydrocarbons. But, I happen to order the crude oil containing 5%...
25 June 2020 9,801 2 View
after miniprep, I sequenced the plasmid. After sequencing I found that a region of plasmid was missing and its size if of around 8kb while it should be of around 12kb. It has happened to me for...
21 March 2020 736 3 View
Hi all, I'm facing problems in staining the cryosection of the cells encapsulated in hydrogels. I'm using both charged slides as well as the albumin coated slides for the HnE staining. During the...
28 May 2019 1,197 5 View
Has anyone done plaque assays for VZV using toluidine stain?
10 March 2019 7,657 1 View
I'm trying to knockdown my protein in adherent cells and label cells with the fucci system for cell-cycle segmentation using microscopy. My protocol is as follows Day 0 : siRNA...
07 August 2018 4,362 4 View
I am currently pursuing Ph.d
01 January 2018 7,222 17 View
Hi all, I wanted to perform ELISA (sandwich elisa) on the protein pellet extracted from cell lysate. I would like to know in which buffer, the protein pellet should be resuspended? Thank you Nisha
10 December 2017 9,325 3 View
Can I get it in matlab format or notepad
08 October 2017 7,788 3 View
My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...
01 March 2021 6,474 3 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...
26 February 2021 7,129 3 View
I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?
25 February 2021 9,761 1 View
I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...
24 February 2021 8,325 1 View