We would like to characterise the antennal sensilla of a moth using TEM. Does anyone have any tips about ultra thin sectioning? I have read several protocols about it, but none of them describe the process in depth. How could I recognise each type of sensillum after I section the segment? (Our moth has seven types). Some papers even mention the approximate region of the sensillum that was sectioned. How could I do do that??
Any help would be greatly appreciated!
It is good to made the serial coups, for exemple 10 + 10 + 10
( for longitudinal coups),
and after photographies all coupes and made in 3D reconstruction
Good day,
Marpha
Do not know about antennal sensilla, but speaking in general, if you can see place of interest in optical microscope you can mount your specimen in a way that allow to cut through it. I prefer flat molds for such task, it's easy to use a microscope to orient a specimen in a proper way in a flat mold. After polymerization a semi-thin sections may be needed for precise location of place of interest.
- Badges
- Science topic
- Similar topics
- Invertebrate Zoology
- Entomology
- Insect
- Neoptera
- Holometabola
- Moths