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Questions related from Ricardo E Castro
We want to characterise some brain regions in an insect using an anti-synapsin protein. Most protocols use Normal Goat Serum to dilute the antibodies and as a blocking solution, but it seems to be...
05 July 2018 9,108 5 View
We would like to characterise the antennal sensilla of a moth using TEM. Does anyone have any tips about ultra thin sectioning? I have read several protocols about it, but none of them describe...
21 February 2018 3,132 3 View
Hi. I have several mass spectra with different values of abundance. Is it possible to normalise all of them, so the scale of abundance goes from 0 - 100%, relative to the base peak? I'm using the...
08 April 2016 6,558 2 View
I was reading an article about how raphides (needle-like calcium oxalate crystals), together with proteins from the plant, are used by some plants to deter herbivory. However, there are plenty of...
07 January 2016 4,446 5 View
I am preparing some samples (external abdominal walls of lepidopterans) to embed them in resin. There are basically two problems: How to recognise which part of the sample contains the region of...
07 August 2015 894 8 View
I have read about pterins, but apart from them, is there any other pigment capable of producing a bright red/carmine colour? Thanks
11 April 2015 8,971 2 View
Are those pigments synthesised de novo, or are they sequestered from their host plants? I'm especially interested in the red pigments found in some caterpillars (e.g. Cossus)
11 April 2015 9,198 3 View
We are starting to use SPME fibres to collect volatiles from plants and other samples, and several protocols mention the fibres should be desorbed from 60 sec to 10 min (depending on the sample...
30 September 2014 6,211 12 View
