Ricardo E Castro

4 Questions 12 Answers 0 Followers

Questions related from Ricardo E Castro

Which is better, NGS or a commercial blocking solution for immunohistochemistry?

We want to characterise some brain regions in an insect using an anti-synapsin protein. Most protocols use Normal Goat Serum to dilute the antibodies and as a blocking solution, but it seems to be...

05 July 2018 9,103 5 View

How to make ultra thin sections of antennal sensilla? Any tips?

We would like to characterise the antennal sensilla of a moth using TEM. Does anyone have any tips about ultra thin sectioning? I have read several protocols about it, but none of them describe...

21 February 2018 3,120 3 View

How to normalise the Y-axis in a mass spectrum, using Agilent MSD Chemstation E.02.00?

Hi. I have several mass spectra with different values of abundance. Is it possible to normalise all of them, so the scale of abundance goes from 0 - 100%, relative to the base peak? I'm using the...

08 April 2016 6,548 2 View

How do insects manage to eat plants with a high content of calcium oxalate crystals?

I was reading an article about how raphides (needle-like calcium oxalate crystals), together with proteins from the plant, are used by some plants to deter herbivory. However, there are plenty of...

07 January 2016 4,441 5 View