In some in vivo patch clamp paper, they often describes a range of resting potential in which the cells are considered healthy and can be used for further analysis. I was wondering how such criteria can be established.
Thank you very much in advance!
Hello Ping, I am not electrophysiologist, but what I saw they try to choose cell to patch which has not interrupted membrane and looks more bright, than the rest. But it is better to ask electrophysiologists
Good luck
If you get a high gigaseal and very low leak current after brake-in you can be sure you have a good cell. You will never get a good gigaseal and low leak with unhealthy cells.
Healthy cells will typically have resting membrane potentials of -55 mV or more negative as a general rule of thumb, and should fire action potentials in response to small depolarizing current injections (100-200 pA). Action potentials should be fairly large and robust (30 mV or more). These are general guidelines, although the exact properties will depend on the cell type. Some cells may also fire spontaneously.
Hyperpolarizing steps of -10mV can be used to check the quality of the seal. Basically, a large current in response to a small voltage step implies a 'leaky' membrane and poor access. Input resistances of 150-200 MOhms are not uncommon, but again, smaller cells might have even higher input resistance.
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