How to make sure that the sequences from PCR product is heterozygous or homozygous DNA and not just sequencing error?

18 January 2013 1 6K Report

I got some heterozygous position in my sequence i.e. R (A/G), Y (C/T) and W (A/T) in my 1 Kb gene product. I will have only two individuals in each species.

Mochamad Syaifudin

I have already got the second sequences of adenosine deaminase from PCR product from another individual. it had the same position of heterozygous DNA. It has16 heterozygous position : 7 synonymous, and 9 unsynonymous. What is the best method to know heterozygous and homozgous DNA variants within individual or species?

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