How to make sure that the sequences from PCR product is heterozygous or homozygous DNA and not just sequencing error?

I got some heterozygous position in my sequence i.e. R (A/G), Y (C/T) and W (A/T) in my 1 Kb gene product. I will have only two individuals in each species.

18 January 2013 5,816 1 View