You need to make a diet containing 80 grams of NaCl per kilogram of the diet. Typical rat chows contain about 10 grams of NaCl per kilogram so a quick-and-dirty method would be to add 70 grams of NaCl to 930 grams of chow and mix thoroughly. Of course, this is not perfect because by adding the NaCl you will slightly decrease the proportion of other ingredients in the diet. There are custom diet manufacturers such as Dyets Inc and Research Diets that make better-controlled diets and they will give advice if you need it.

Thank you for your answer michael tordoff?

How much gram of high salt diet per body weight of rat?

That will depend on how much your rat eats, and that depends on its age, sex and strain, and the formulation of the diet you give it. For rough calculations, a typical young male adult rat will weigh about 300 - 350 grams and consume around 20 grams of chow a day (i.e., about 60 g/kg body weight per day). This is equivalent to 1.6 g NaCl per 325 g body weight per day or about 5 g of NaCl per kg body weight per day.

As it has been told by Michael Tordoff adding salt to diet (chow) is not perfect because itwill decrease the proportion of other ingredients in the diet.I think adding salt to rats drink water will be more easy and feasible way to salt intake by animals.

Don't add salt to the drinking water. Low concentrations (below 150 mM) will not kill the rats but they will consume at most 40 ml/day so they won't be exposed to much NaCl. Water containing anything above 150 mM NaCl (isotonicity) will cause dehydration and death, much the same way as marooned sailors will die from drinking seawater.

i get from journal to add about 2% body weight of rat with 8% NaCl via drinking water, will make hypertensive in rat from this journal "Antihypertensive effect and eNOS expressions in naclinduced hypertensive rats treated with purple sweet

potato",

Can i implemented in fibrosis in rat with 2% body weight of rat with 8% NaCl in 2 months according from journal "Salt Induces Myocardial and Renal Fibrosis in Normotensive and Hypertensive Rats" ?

I don't understand the question. Obviously, if you follow the methods of a published paper then you should get the same result.

I took a quick peek at the sweet potato paper and I don't see any mention of how the authors delivered NaCl to their rats. Perhaps you should e-mail or phone the author. The contact info is on the title page.

i get 2% of NaCl drinking water from body weight from this journal, is this can be done?

Hi Ricky, so do you manage to induce hypertension to your rats using the methods mentioned in the article you attached above?