There are few steps to make heatmap of your qRT-PCR data (fold change or relative quantification) using R.
Data file preparation:
Make excel file of your data in which your will place your gene of interest in column and your treatment or conditions in row.
Save the file in *csv extension.
Import data file in R:
By using following codes, import your data file into R,
data2
See these links:
https://www.researchgate.net/post/How-to-make-a-heatmap-in-R-from-a-PCR-data
https://www.researchgate.net/post/What-is-the-proper-way-to-transform-and-normalize-large-qPCR-datasets-for-statistical-analysis-and-heat-map-visualization
https://addi.ehu.es/bitstream/handle/10810/21789/Bachelor_Dissertation_NachoMachado.pdf?sequence=1
https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0014820_RelativeQuant_UG.pdf
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