This paper may be of interest

Methods Mol Biol. Author manuscript; available in PMC 2008 June 9.

Published in final edited form as:

Methods Mol Biol. 2007

Aging Cell Culture

Methods and Observations

Sharla M. O. Phipps, Joel B. Berletch, Lucy G. Andrews, and Trygve O. Tollefsbol

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2423218/

Culturing and subcultivation of normal human diploid fibroblasts have advanced our understanding of the molecular events involved in aging. This progress is leading to the development of therapies that slow or ablate the adverse physiological and pathological changes associated with aging. It has been established that normal human diploid fibro-blasts can proliferate in culture for only finite periods of time. Hayflick and Moorhead and others have described numerous types of cells, ranging from fetal to adult, that were incapable of indefinite proliferation. There are many ways to study aging in vitro, and this chapter summarizes some laboratory procedures.

Good luck

You could expose it to higher levels of Oxygen! Luckily this is very easy to do, just place them in a standard incubator and culture them like everyone else. Everyone is working with cells which have ROS profiles that are through the roof and sadly enough most people don't even understand that no cell in the body sees 21% Oxygen.

This paper may be of interest

Methods Mol Biol. Author manuscript; available in PMC 2008 June 9.

Published in final edited form as:

Methods Mol Biol. 2007

Aging Cell Culture

Methods and Observations

Sharla M. O. Phipps, Joel B. Berletch, Lucy G. Andrews, and Trygve O. Tollefsbol

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2423218/

Culturing and subcultivation of normal human diploid fibroblasts have advanced our understanding of the molecular events involved in aging. This progress is leading to the development of therapies that slow or ablate the adverse physiological and pathological changes associated with aging. It has been established that normal human diploid fibro-blasts can proliferate in culture for only finite periods of time. Hayflick and Moorhead and others have described numerous types of cells, ranging from fetal to adult, that were incapable of indefinite proliferation. There are many ways to study aging in vitro, and this chapter summarizes some laboratory procedures.

Good luck

I understand you are trying to age the cell line whether by generation or otherwise . My apologies for my slight ignorance and near sarcasm above as it was an attempt to make people think twice about our obsession to ignore oxygen. The earth was flat... and Oxygen wasn't important in the dish... that's how I look at it.

This is interesting, the link provided above completely ignores Oxygen and doesn't mention oxidative cellular damage / ROS? I read copious amounts of research focused around Oxygen, macrophage dysregulation, Oxygen/Redox Homeostasis and much more. Between the CELLS ENERGY, inter and intracellular signalling, senescence & apoptosis, angiogenisis and it's involvement in almost every disease and I just can't understand how we are still culturing cells without Oxygen? HIF is pleiotropic, mir210 is pleiotropic. My apoligies for taking this slightly off topic but can someone shed some light on this for me?

Thank you, everybody. I just want to age my primary cell quickly. Brad's idea impressed me very well. It seems that Oxygen is a good idea. I think I will have a try.

You can expose them to oxidants such as H202 or create a line of cells specifically for this purpose by knocking down one of the genes involved in reducing ROS arising from mitochondrial activity.

What if you could control your incubator by what is happening in the dish? There are solutions available to do this, message me and I share a couple options.