Hi everyone, have you aver used K-PIPES buffer? how to prepare it and in case, is there any other commercial buffer that I can use for fixation?
These numbers check out; check math and try this at small scale initially to be sure it works!
for 1L 0.8M K PIPES, pH 6.5
241.896 g/L PIPES free acid
600 mL diH2O, 2.9 pH initial
stir and slowly add
112 mL 10M KOH
PIPES will not dissolve fully until pH -> 6.5
add diH2O to final 1L volume
Thank you Edward :)
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