Blerta S.

3 Questions 3 Answers 0 Followers

Questions related from Blerta S.

Detect mRNA and protein on the same sample-combination of HCR and IF in cells?

I am working on setting up a protocol to detect mRNA and protein on the same samples. I know that people have done that in zebrafish and other tissues but I was wondering if you have any...

20 September 2024 4,973 1 View

How to make 0.8M K-PIPES buffer ph6.5?

Hi everyone, have you aver used K-PIPES buffer? how to prepare it and in case, is there any other commercial buffer that I can use for fixation?

20 April 2022 2,341 2 View

I am studying DNA damage in cancer cells, I measure yH2AX with HTS (high throughput screening) but I have low signal. Can you help me?

I am doing immunofluorescence in cells treated with Camptothecin. I measure yH2AX foci. Every time that I repeat the experiment I obtain like 5-8% which is very very low if I compare with other...

26 June 2016 4,263 3 View