We (= Evolution Lab of Faculty of Science in Zagreb) have collected fresh samples of swamp water from bog in continental Croatia a few days ago in which we found a lot of Paramecium bursaria specimens. Since this ciliate species is very interesting for research and for teaching students on endosymbiosis, we are interested where to find information, published or unpublished protocol how to maintain acidic medium similar to that in bog in lab conditions (I assume it is by adding some swamp-similar buffer and it would be very nice to know its compounds). We have gotten info that P. bursaria is very in the long run, we need something better. Thank you for your time and answers in advance.
Josip:
Kindly see this link and references therein for insights:
Best
Syed
Dear Josip, my experience with this species is connected with medium of cabbage solution. Should take a heard of somewhat decayed c. Couple g. (2-4) per l is enough. Should exp with proportion. Anyway this exp. is not of purely scientifically approach - just field experience. As well good light is necessary. All this is following by the very bad small!!! Try...
For genetically pure culture should call may be Wilhelm Foissner or Bill Bourland labs.
Andrey
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