plz use some specific fungicides in media ........eg we use benomyl to suppress the chytirid (oomycetes) for growing other fungi. In other ways the Rose bengal is good source to limit every colony of fungi on media plate.

Thanks ahmed, here we had the rose bengal agar it shows the mixed fungi, but you said to avoid the sporing fungi use the benomyl. How we get benomyl ,kidly explain about it .

Sekran: rose bengal restricts the fungal colony to spread u can get your soil fungi seperately after getting different colonies. Then after these may be separately transferred to a media plates alone, either these are sporing or sterile hyphae. If u want to suppress the group of fungi plz find specific fungicides and add it in a medium. Benonyl is fungicides that suppress most fungi but doesnt suppress the pythium, albugo, phytophtora, etc.

Thank for your kind help

Restricted fungal growth in Rosebengal supplemented medium.

If you want to isolate many fungi of roots and soil, you´ll need also add to the culture media some antibiotic such as (Chloramphenicol and Cycloheximide) to avoid bacterial contamination, the latter one also could be reduce the contamination by moulds and yeasts.

Dear Karthikeyan,

Your question is not fully clear to me. Is there a particular reason why you want to isolate wood degrading fungi (white rot fungi - WRF) directly from the soil? Do you aspect to have many litter degradig fungi as well? In any case... either using Benomyl or Rose Bengal the effect is the same, you will have a reduced growth of all fungi. Also the suggestion of using antibiotics in your media is correct since you might aspect a long time incubation for those non-sporulating or for the WRF, thus it will inhibit the spead of unwanted bacterial contaminations.

From my experience I would suggest you to split the screen in two parts: 1 WRF, 2 other non sporulating fungi. For the second part the best is to use growth inhibiting chemicals, but for the first part (WRF) might be better to use a synthetic media in which the only C source will be available to those fungi able to degrade cellulose or lignin (i.e. carboxymethyl cellulose CMC or lignin sulphonate). Adding a second step to this screen by using RBBR should gice you a good indication whether the isolated strains have ligninolytic properties.

Hope this might help

Best regards

Which form of WRF do you expect? Mycelia, rhizomorphs or spores? From mineral soil of from above ground humus layer? Qualitative or quantitative assesment?

Some kind of extraction mycelia or rhiz.. from supernatant would be usefull to avoid large deal of other fungal particles..

Hello Karthikeyan

if you find symbiotic fungi( mycorrhiza and endophyte), I believe, need a work with a FIM media or minimun media(with oats 10g. ), and them perform a field by serial dilution of the soil, and then when he grows any Colony move another petri dish. Because some symbiotic fungi grow very slow.

best wishes in your work.

Julio

Check literature to find a selective medium for your species, or use baits which are also reported in literature for a number of soil fungi

For non spore producing fungi use agar coated slides and insert into the soil sample or go for meta genomics studies

I usually use a selective medium for the isolation and quantification of the white rot fungus (P. chrysosporium) from soil. Please see the suggested medium and protocol in the attached file (p 39/40).

Have you resolved the problem? We found that low quantities (0.1 %) of Carbendazim inhibited most of mitosporic fungi. Thus you can add it to your culture medium. You can use it also with antibiotics.