Hi all, I am new to the computational suite"MolPro" and I am trying to do the Electronic energy calculation for a small system. Following is my Input...
29 March 2024 1,178 2 View
Through my preliminary experiments, it was found that there is an emission of abnormal ultra-high energy electrons downstream of the RF cavity of the electron storage ring, which I theoretically...
12 March 2024 8,573 1 View
What should be the temperature for PCR machine lid during ligation overnight at 16c? Will the usual lid temperature at 105c affect the ligase performance?
20 February 2024 7,765 3 View
I made two 10% sds-page gel (10% resolving and 4% stacking) and run them at 20mA. I am testing whether my newly edited recipe for gel casting is correct or not. Apparently, the gels took 3hr for...
13 December 2023 7,487 1 View
I am optimising a new protocol for SDS-PAGE using 40% acrylamide 29:1 solution from Bio-Rad across different percentages. I casted two 6% equivalent gels according to the protocol I have...
08 November 2023 7,022 0 View
please recommend best markers for eosinophil. Thanks.
05 September 2023 7,938 0 View
Atoms of SiC material, one silicon atom will connect four carbon atoms, is the bonding energy between this silicon atom and four carbon atoms the same?
23 July 2023 9,880 5 View
I am thinking of sorting Ter119+ cells from the spleen and bone marrow to look at the transcription profile in immature RBCs and erythropoietic progenitors. Could I use RBC lysis to remove mature...
06 July 2023 7,826 0 View
I want to use FDTD to simulate black phosphorus absorption A. Since black phosphorus is not in the material library, I add 3D new material by looking for the permittivity. The references are...
01 June 2023 5,739 1 View
I plan to check t cells from mouse tumors for IFN-y, TNF-a, IL-2 and granzyme B. Is T cell activation by PMA or anti-CD3/CD28 needed? Thanks!
31 October 2022 2,634 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I need a very narrow glass capillary for my research. How can I make a glass capillary with an inner diameter of around 200 to 500 nanometers?
08 August 2024 8,915 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View