04 May 2023 0 2K Report
  • I've used to isolate LSECs several times with percoll before, but all failed. I'm looking for someone here with relevant experience to give me some guidance.
  • If I get useful suggestions, I'll give you a reward! Thank you very much!

More Qian Jialong's questions See All
How can I remove the problem occurs in ciorth in Molpro calculation?

Hi all, I am new to the computational suite"MolPro" and I am trying to do the Electronic energy calculation for a small system. Following is my Input...

29 March 2024 1,178 2 View

Do you know that there is the emission of ultra-high electrons with energy up to 790 TeV downstream of the RF cavity in the electronic storage ring?

Through my preliminary experiments, it was found that there is an emission of abnormal ultra-high energy electrons downstream of the RF cavity of the electron storage ring, which I theoretically...

12 March 2024 8,573 1 View

Temperature for PCR machine lid during ligation?

What should be the temperature for PCR machine lid during ligation overnight at 16c? Will the usual lid temperature at 105c affect the ligase performance?

20 February 2024 7,765 3 View

Why protein ladder starts separating in sds-page stacking gel?

I made two 10% sds-page gel (10% resolving and 4% stacking) and run them at 20mA. I am testing whether my newly edited recipe for gel casting is correct or not. Apparently, the gels took 3hr for...

13 December 2023 7,487 1 View

A line appeared on the SDS-PAGE gel, what is the cause?

I am optimising a new protocol for SDS-PAGE using 40% acrylamide 29:1 solution from Bio-Rad across different percentages. I casted two 6% equivalent gels according to the protocol I have...

08 November 2023 7,022 0 View

Eosinophil cell marker?

please recommend best markers for eosinophil. Thanks.

05 September 2023 7,938 0 View

SiC atomic bonding energy same?

Atoms of SiC material, one silicon atom will connect four carbon atoms, is the bonding energy between this silicon atom and four carbon atoms the same?

23 July 2023 9,880 5 View

Are immature RBCs and erythropoietic progenitors resistant to RBC lysis?

I am thinking of sorting Ter119+ cells from the spleen and bone marrow to look at the transcription profile in immature RBCs and erythropoietic progenitors. Could I use RBC lysis to remove mature...

06 July 2023 7,826 0 View

FDTD simulation of black phosphorus absorption?

I want to use FDTD to simulate black phosphorus absorption A. Since black phosphorus is not in the material library, I add 3D new material by looking for the permittivity. The references are...

01 June 2023 5,739 1 View

Does flow cytometry checking t cell cytokines from tumor-bearing mice need t cell activation?

I plan to check t cells from mouse tumors for IFN-y, TNF-a, IL-2 and granzyme B. Is T cell activation by PMA or anti-CD3/CD28 needed? Thanks!

31 October 2022 2,634 4 View

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Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

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Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

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How to use Density Functional Theory to calculate carrier mobilities of solid system?

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Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

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Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

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Best mask design for metal deposition aiming build a Perovskite solar cell device?

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