How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

What are optimal centrifugation settings for murine eosinophils?

Hi everyone! I am currently trying to differentiate and culture murine eosinophils from bone marrow cells (with FLT3-L, SCF, and IL-5) according to Dyer et al., 2008. After 18 days in culture I...

22 March 2020 5,840 3 View

Does anyone know a specific marker for identifying eosinophils and basophils using flow cytometry?

I will be using human whole blood samples.

06 January 2020 3,767 3 View

Does anyone know a specific marker for identifying eosinophils and basophils using flow cytometry?

I will be using human whole blood samples.

31 December 2019 3,647 2 View

How can I detect level of eosinophils in blood by ELISA method ?

I want to check eosinophil level in the mice blood/serum, for my experiment, using ELISA kit. need your advice, Thank you !

17 September 2018 1,985 5 View

How can I detect level of eosinophils in blood by ELISA method ?

I want to check eosinophil level in the mice blood/serum, for my experiment, using ELISA kit. need your advice, Thank you !

08 September 2018 242 6 View

What cell types can I identify with my flow cytometry panel?

20 May 2018 9,335 3 View

What cell types can I identify with my flow cytometry panel?

04 May 2018 4,766 3 View

Hello everyone. How can I stain the sputum smear ?

hi I am PhD student in my research protocol i need to count non squamous cell in the sputum. I'm currently using the May Grunwald's Giemsa ( MGG) but eosinophil granule not stained pink.....

02 March 2018 9,892 3 View

Best blood dye for eosinophile count?

Hi Guys, I am currently planning my field work for my master's degree where I am looking at immune response by measuring eosinophiles. Are there anyone out there who might have a tip to a dye...

31 December 2017 4,750 6 View

Anybody experienced with unspecific staining of eosinophils in FACS?

Hey guys, I am trying to stain eosinophily for intracellular proteins. First step ist to stain them with fixable viability dye, after that fixate and permeabilise them to stain for intracellular...

08 September 2017 3,517 3 View