What is the best method to explore the resistance gene of trypanosoma?

I have a research to find the resistance gene of Trypanosoma evansi to diminazyne drugs. Well, there are two type of mthode that my supervisor offered to me, by using the RFLP-PCR or RT-PCR, but...

09 October 2017 4,691 4 View

Is anybody know why Control well (without antigent) on ELISA is always giving a titter of OD?

My final thesis is about meassuring the OD of IgG From sera which reacted with antigen of malaria vector (An.sundaicus) by using ELISA, but I got problem when the control negative (without...

02 March 2015 7,743 6 View

Does any one know about the normal titer of IFN Gamma and IL-10 ?

02 March 2015 4,824 4 View

Any advice or method on how to make artificial Feed of Mosquito?

An. sundaicus is one major vector of malaria in java, we want to rearing this species in our laboratory but there's a problem with mosquito's blood feed, in our laboratory us mice (wistar) as...

05 June 2014 3,463 3 View

How do you change the concentration of the antigen to run ELISA?

The protein stock I have is 2 mg/ml. I want to make the concentration is 4 mg/50 ml. Can anybody help me?

02 March 2014 1,738 4 View

How to isolate genomic DNA from marine bacteria?

I tried to use the freeze and thaw method but it's not working. My friend who work with same method did it as well, but his bacteria is from soil.

02 March 2014 4,688 9 View

Any advice/information/recommended literature on the ELISA Technique?

I am researching the reaction anti-salivary Gland of Anopheles in human antibody using the ELISA technique.

10 November 2013 2,137 6 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View