I've been trying to isolate total RNA from guinea pig's knee cartilage. I've tried Qiagen RNeasy kit, miRNeasy kit, trizol method with lots of modifications and have also used the RNaqueous total RNA isolation kit from Thermo Scientific but I've not been able to achieve RNA ratio of more than 1.4 for cartilage and 1.6 for synovium. In addition to the integrity the RNA conc. is also very low because the amount of cartilage available from the knee of guinea pig is very less (
Sandeep Kumar
Hi Dikshit..because your starting material is less so, in Trizol method u can do overnight precipitation of RNA in isopropanol at -80°C as it will improve quantity as well as quality.
Hope it will work.
Thank u.
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