I am looking to isolate Pancreatic islets from C57BL/6J mice using Ficoll Paque Plus and also Percoll. Can anyone mentioned the actual steps needed to isolate the islets? Thank you.
Will there be a change in bandgap energy of AgNP exposed under blue, red and visible wavelengths of light?
22 March 2024 2,317 2 View
My Xcrysden crashed and I was searching for the solutions. Finally, after several attempts I reached a solution of the same. Follow these steps if your xcrysden crashes with the error "(file...
11 January 2024 3,375 3 View
Hello everyone, I am doing a calculation with QE version 7.2 and thermo pw version 1.8.2. The files running fine with the quantum espresso but giving error while running it with thermo pw....
29 November 2023 6,289 5 View
I have a few proteins that I produce recombinantly in E. coli cells. All of them are disordered proteins and are stored in the presence of 4mM DTT at -20°C before use. One of them also contains...
28 November 2023 2,682 2 View
In the western Himalaya there are metabasic volcanics said to be intercalated with metasediments of supposedly proterozoic age. The metasediments are intruded lower Paleozoic granite. The granite...
01 October 2023 7,892 2 View
Hello Everyone, Iam planning to buy a new laptop and checked one laptop with i5-13500 processor which is having 12 processor but 4 performance cores and 8 efficient cores. So can I use all the...
30 September 2023 7,634 4 View
I am searching for equivalent keyword in ORCA for "optrot" that is used in Gaussian.
19 June 2023 9,726 2 View
We want to investigate the solid phase of 1-propanol using Molecular Dynamics, and for that, we are looking for the experimental crystal structure of this molecule (1-propanol) to use as the...
08 June 2023 1,599 0 View
Hello Everyone, I am able to sucessfully run scf.in using pw.x but while proceeding for the calculations to be done using thermo_pw.x the following errors occur. Error in routine c_bands...
15 November 2022 3,734 2 View
Using the Boltztrap and Quantum espresso I was able to calculate the electronic part of thermal conductivity but still struglling for the phononic part of thermal conductivity. I tried the...
02 November 2022 2,853 6 View
Hi y'all, does anyone know if animal cells increase in size as they age or do they simply multiply? I usually do a Percoll gradient centrifugation to isolate endothelial cells but with pups it...
07 June 2024 4,065 1 View
I was wondering if the plasma that is in the top layer after centrifugation can be used to assess proteins, metabolites, hormones, or other molecules, or if the ingredients of the Ficoll alter the...
03 June 2024 1,852 4 View
I'm not sure how to interpret or fix the sample so that it creates a normal band. I used Percoll Gradient with density gradients to generate the questionable band. It's supposed to represent...
14 April 2024 9,350 1 View
Hello all, I present here a problem to see if someone has encountered it and can provide some clarity. We are trying to isolate neutrophils from patient blood in tubes EDTA. We are following the...
10 March 2024 4,156 3 View
Hi, I am using 2 gradients Percoll protocol to isolate neutrophils from whole blood. I prepare gradients of desired density myself on the day of the experiment from Percoll (Sigma 1644). In my...
28 February 2024 9,499 1 View
I'm trying to isolate Lysosome using a percoll (sigma, cat# 4937) gradient of 57.6%, 43.2%, 28.8% and 20.2% (Ref: Lysosomal and non-lysosomal peptidyl hydrolases of the bloodstream forms of...
21 February 2024 1,028 1 View
Could ficoll be used as a substitute for percoll in dencity gradients when performing microglial isolation? Right now, we are trying to isolate microglia for flow cytometry. We are going to use...
24 January 2024 1,534 0 View
Hello, I would like to know the concentration of collagenase that dissolved in P2 perfusion (Hepatocyte organoid) This is because the liver does not break down easily and there are not many live...
04 January 2024 7,835 2 View
For isolation of PBMC, at what temperature we should keep sucrose solution. Which method is better for PBMC isolation? Ficoll DGC method or Sucrose DGC method?
26 November 2023 3,726 2 View
Hi everyone, We've tried to perform beta cells isolation form murine pancreas unsuccessfully. We used Samaddar(2020)protocol, using collagenase XI digestion and Ficoll gradient centrifugation. We...
14 November 2023 2,558 2 View