Snehasish Nag

2 Questions 4 Answers 0 Followers

Questions related from Snehasish Nag

How to isolate pancreatic islets from C57BL/6J mice using Ficoll Paque Plus and Percoll?

I am looking to isolate Pancreatic islets from C57BL/6J mice using Ficoll Paque Plus and also Percoll. Can anyone mentioned the actual steps needed to isolate the islets? Thank you.

27 January 2022 9,406 0 View

Can I perform islet isolation after storing the excised mouse pancreas for 3 months and what is the protocol for this process?

I have sacrificed treated BALB/C mice and collected the pancreas and stored in -20 degree centigrade for 3 months. Now I want to isolate the islets to study the release of different cytokines from...

09 October 2020 7,519 6 View