How to isolate hepatic adventitious fibroblast by Percoll?

15 April 2025 0 3K Report

I am trying to isolate hetatic adventitious fibroblasts (AF) from mice, still not so sastisfied with my results. I tried either with 33, 40 or 45% Percoll, top up with HBSS to seperate leukocytes and AF. Leukocytes pelleted well, but my AF band was unclear, mostly floated with fat/debris foam. Does anybody have protocol or trouble shooting tips? Any share is highly appriciated :)

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