How to isolate cancer cells and immune cells from tissue?

More Wnhidayah Wh's questions See All

What are the advantages of self-assembly & amphiphiles nanocarriers vs hard nanoparticles?

what are the advantages of self-assembly & amphiphiles nanocarriers vs hard nanoparticles for drug delivery ?

03 April 2019 4,823 8 View

How polymersome/nanocarrier can carry the drugs and target specific cancer site?

Hi everyone, I am looking for effective way on how polymeric counterparts of liposomes (polymersome)/the organic nanocarrier can be efficient transport of drugs molecule targeting specific cancer...

03 April 2019 334 4 View

What different between colon cancer and colorectal cancer? Some put colorectal cancer as colon cancer. Re anatomy, there's different.?

01 February 2019 6,805 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Karen A. Darbinyan Popular answer

Hello, see below info, may be useful for you.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3471300/#__ffn_sectitle

https://www.labome.com/method/Cell-Isolation-and-Separation-Techniques.html

https://www.dls.com/biopharma/blog/5-cell-isolation-techniques-to-consider-for-cancer-research

Hope this info will help you!

Good luck!

Karen A. Darbinyan

Wnhidayah Wh

very useful link. Thank you Karen A. Darbinyan , Jiang Kunpeng