I utilize dialisis for release determination with PBS (pH 7,4) + surfactant (1% PVA). After about 2 hours a precipitate was formed in dialisis bag (I believe this was my released drug). So I decided to measure drug content in precipitate after centrifugation. But the results were quite strange: there was a fluctuation of values of drug concentration. What do i need to change in my experiment?
You need to provide conditions where the drug does not precipitate. Use another surfactant (a small one) and increase the volume you are dialyzing against. I also prefer not using dialysis bags since the membrane will interfere with the actual release from the plga particles.
Thank you.
Is it permissible to use a mixture of organic solvent and PBS?
The organic solvent might dissolve your plga particle or accelerate your drug release by changing the structure of the plga particle
For this purpose a solvent is required that dissolves the drug but not the polymer, e.g., alcohols such as methanol, to be able to extract a hydrophobic substance from PLA nanoparticles within 24 h without dissolving the polymer or other solvents might be useful for ur case are methylene chloride, acetonitrile, acetone, tetrahydrofuran DMSO, or 1,4-dioxane as I hv seen in literature so far....
Do not use organic solvents even if they do not dissolve the polymer, they will enter the particles affecting the diffusivity of the drug, hence the release.
Dear Omar,
Yes u r right...my explanation cn be just used for determining of drug amount in precipitation after centrifuge n not for drug in nanoparticle,,,,
The drug release can be determined either directly or indirectly. In this case, considering the difficulty to measure the released drug, you may also quantify the remaining drug in your nanoparticles.
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