What modeling software can be used to analyse fate of pesticides using a fugacity multimedia approach?

Hello, I am trying to understand the fate of pesticides based on the fugacity multimedia approach. Is there software for such modelling? I know SWAT can be used for watershed-level pesticide...

15 October 2020 1,424 3 View

How to amplify extremely high GC-rich template?

I am cloning a whole cDNA of a particular gene and I have designed forward primer at start of 1st exon. The region downstream of forward primer is extremely high GC rich (around 85-89%). Because...

17 September 2020 6,571 11 View

How to apply load in abaqus ?

I have created a beam and i dont know, what is the maximum load it can take, Is there any way to increase the load continuously without giving a fixed value, so that at some load the beam will...

02 September 2020 6,246 9 View

I have a list of properties of tool steels here. Please let me know which of the below mentioned properties will change after heat treatment?

16 August 2020 730 8 View

How to define hyper elastic UMAT in a UEL?

Hi all, I am working on Phase-Field UEL for hyperelastic materials. I am using codes of Dr Molnar ( http://www.molnar-research.com/tutorials_videos_3.html) to develop hyperelastic material. I...

07 April 2020 4,243 1 View

Related to Jasco FP-8300 spectrofluorometer?

I used Jasco FP-8300 spectrofluorometer to take a spectra of riboflavin but I got two instead of one. I had changed different solvent but the spectra remained same. Is this instrument fault or...

15 September 2019 633 1 View

What's going wrong with plastic sectioning?

I am doing Aniline blue staining in rice anthers to detect callose signaling. When anthers are squeezed and stained, I could detect the signaling. However, when I do plastic embedding with...

10 April 2019 9,131 15 View

What would be the formula for finding the stretch force for expanding an O ring made of elastic ? On what factors the expansion stretch force depends?

I want to expand an O ring made of elastic material and am looking for the mathematical expression (dynamics) on which the stretch force depends. In addition to this, how does it vary in different...

18 March 2019 4,158 3 View

Getting fewer colonies on SD-gal plates when compared to glu plates.Need help with increasing the transformation efficiency on gal plates?

Hello , I transformed yeast cells with a plasmid containing URA marker and plated on SD-Glucose and Galactose plates.I obtained about 350 colonies on the glucose plates but only 100 on galactose...

10 September 2017 9,431 8 View

Bacmid DNA is not getting dissolved in TE buffer

Hi.I am facing problems in the bacmid isolation step.Currently i am using purelink hipure plasmid miniprep kit for isolating the bacmid.But the problem is,after the isopropanol-ethanol step when i...

13 August 2017 1,069 10 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

How to measure pH in anaerobic digester?

I'm planning on doing research by using anaerobic digester with liquid substrate and want to see its pH changes periodically. Any suggestion on measuring the pH with maintaining its anaerobic...

09 February 2021 1,293 1 View