How to check the pH of E. coli culture initially? and how to change the pH of the E. coli culture?

I am working on E. coli 15597 prepared in Luria broth. Initially, the pH of LB is 7 after the inoculation of the E. coli it changes to 8.26 is it correct? can bacteria be alkaline?

15 October 2020 2,042 2 View

What are the best pipleline available for the RNA-Seq data analysis ?

What is the latest pipeline to run the RNA-Seq genomic data ?

12 October 2020 7,618 2 View

If an article is unknowingly published in a fake journal, is it considered as published or can the article be sent to some other journal to publish?

How do we handle this menace of fake journals?

14 July 2020 4,835 7 View

How do we differentiate conservation agriculture and regenerative agriculture in context of sustainable farming practice?

Sustainable farming practices appears into different names confusing the global audience which to follow and why? What makes conservation agriculture as different approach than regenerative...

30 June 2020 7,726 2 View

How to get DOI for my published article in a journal which has not given any DOI?

I want to know if Research Gate generates DOI for articles which has been published but has not been provided any DOI by the publisher?

23 May 2020 510 7 View

How many times do we have to check and report model fit measures during SEM?

I am trying to run a SEM which has four latent variables in it. Let us consider these variables as A, B, C and D. All are latent. Here A and B are independent variables, C is mediator and E is...

17 May 2020 4,091 3 View

What is the criteria to calculat e the plagarism?

What is the criteria to calculat plagarism for writing manuscript

06 April 2020 6,936 3 View

What happens if we lower the number of cycles in explicit dynamics for ansys ?

Hi I am a student pursing my final year in mechanical engineering. I am doing crash analysis of the car frame in Ansys software in explicit dynamics module. I had my analysis running for 2-3 days...

18 March 2020 3,709 3 View

Why contaminant is transporting from lower part to higher part in various column experiments? What is reason behind this?

can we transport the contaminant from the higher part to the lower part of the column?

25 December 2019 7,124 3 View

Can authentication in IoT be implemented using BlockChain techniques?

Authentication in IoT systems are vastly insecure. Mostly the problems lies on its open ended and probable untrustworthy nodes. Also, the need for a lighter authentication mechanism will always be...

09 December 2019 4,896 4 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

How can I verify my centrifugal fan design for electric motor cooling?

I have radial blade centrifugal fan design which will be mounted on exhaust side of electric traction motor. The fan will be mounted on same motor shaft inside the housing. How can I verify that...

23 February 2021 2,390 3 View

Thermal reduction of graphene oxide.?

I am trying to reduce graphene oxide by thermally but I have no proper information regarding this. Can any one help me regarding the details about temperature and time for the reduction of...

23 February 2021 4,155 4 View