I want to check the growth phenotype of a population of yeast segregants, but I have a trouble with starting the cultures. If i start from glycerol stocks, all the cultures do not get revived.
Please suggest the appropriate solution for the above query
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I am trying to amplify a gene for a mouse histone linker protein using PCR for cloning purposes. For this, I have used cDNA synthesized from RNA isolated from mouse liver as template, but I am...
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Strangely, my yeast colony PCR works only with Sapphire hot start master mix or Amplicon master mix but not by any other master mix, or if I individually add Taq polymerase with compatible buffers...
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Part-1: Recrystallization kinetics is given by JMAK equation which is written as Xᵥ=1- exp(-Btᵑ ), where B=fṄĠ^3; Ṅ= rate of nucleation; Ġ = Velocity of high angle grain boundaries which is...
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storing TBS-T for some time results in some cloudy precipitates. Is it still usable
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I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
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I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
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Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
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Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
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I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...
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I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
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I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...
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In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
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I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
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Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...
01 August 2024 2,250 2 View