Purnima Kovuri

2 Questions 1 Answers 0 Followers

Questions related from Purnima Kovuri

Why does yeast colony PCR not work with all master mixes?

Strangely, my yeast colony PCR works only with Sapphire hot start master mix or Amplicon master mix but not by any other master mix, or if I individually add Taq polymerase with compatible buffers...

04 November 2020 4,004 3 View

How to inoculate yeast in 96 well plate to phenotype growth?

I want to check the growth phenotype of a population of yeast segregants, but I have a trouble with starting the cultures. If i start from glycerol stocks, all the cultures do not get revived.

16 June 2019 5,093 0 View