I am inoculating bacteria and fungi onto separate wood blocks. I know how to do the fungi, the bacteria I am struggling with as there appears to be different ways. I was going to grow bacteria in nutrient broth, measure OD of cells, the spin it down, decant the broth then repeat using Ringers twice to wash them. The resuspend in Ringers and but 2 mL into a bijou tube and put the wood block in the bijou tube for a period of time to allow bacteria to colonise. Does that sound like a viable option and if I leave it for a month will it affect the wood or the bacteria regarding oxygen in the Ringers?
Thanks, that involves submerged wood so putting them in water would make sense as it's their natural environment. Mine is just beech blocks being inoculated with fungi and bacteria that have been found in the wood louse guts. I'm using Ringers as it will protect the microbes better than water
If I am going to keep the cells at 4C they need to be preserved and they wouldn't be viable if kept in water
Hello Caroline
Several useful references on attachment 1
https://books.google.com/books?isbn=1780647123
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https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=12&cad=rja&uact=8&ved=0ahUKEwj81sO3lpHWAhVIVxoKHWVwAq8QFghGMAs&url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles%2FPMC4732817%2F&usg=AFQjCNEgBz-0Tegwd-XFj425vdH37N0bgg
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http://svenska-forpackningsforeningen.se/assets/svenska_forpackningsforeningen/archive/dokument_lankade_publik_sida/schonwalderwoodenboards.pdf
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https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=12&cad=rja&uact=8&ved=0ahUKEwidnI6rl5HWAhWClxoKHad9DJs4ChAWCC8wAQ&url=http%3A%2F%2Fwww.treenshop.com%2FTreenshop%2FArticlesPages%2FSafetyOfCuttingBoards_Article%2FCliverArticle.pdf&usg=AFQjCNFt5hMPTHcasK7-SoXIuKML90yeMQ
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https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=18&cad=rja&uact=8&ved=0ahUKEwidnI6rl5HWAhWClxoKHad9DJs4ChAWCFIwBw&url=http%3A%2F%2Fwww.sciencedirect.com%2Fscience%2Farticle%2Fpii%2FS1049964401909533%2Fpdf%3Fmd5%3D78bee3af56dda0387d989349df8c8927%26pid%3D1-s2.0-S1049964401909533-main.pdf&usg=AFQjCNGD8ASLfyy2w6K_Pfvn4rcddYUk1A
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https://journal.frontiersin.org/article/10.3389/fmicb.2015.01137/full
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http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.956.3900&rep=rep1&type=pdf
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https://onlinelibrary.wiley.com/doi/10.1111/j.1574-6941.2007.00425.x/pdf
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https://pdfs.semanticscholar.org/3edf/99f9f231ed56023391d0382c80260c0f517a.pdf
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