I am new to extracting RNA from fruit flesh. Currently, I'm extracting RNA from sweet cherry flesh using Fruitmate and Nucleospin Takara Bio. I have tried using RNeasy kit Qiagen but I failed (but with another fruit sample I can extract it).
For shredding the tissue I used multibeads shocker and store the sample at -80 degree celsius freezer.
I have tried to follow their instructions and try to create a clean workspace as possible. My problem is that the extraction always resulted in low concentration (both 1x and 2x elution) and absorption is also very small (0.xxx of A260 and A280), also A260/A280 ratio of 1.5 - 1.89. I want to know where did I do wrong but I don't know how to evaluate it. Please help.
Why dont you try the Trizol method with crushing the sample in liquid nitrogen and further processing
Thank you for the suggestion Henry Kolge, I finally tried again using more samples in one column of NucleoSpin RNA and it works
- Badges
- Science method
- Similar topics
- Biological Science
- Histology
- Staining