Dear Researchers, I am trying to induce Motile Aeromonas Septicemia in Loach (15 gram)fish by injecting fish pathogenic strain of Aeromonas, injecting intra- muscular,at different doses 10^6 ,10^7. But there are no sign of disease (7 days)

The water temperature is 26c .Fish were kept without any feed, 3 days prior to experiment to induce the stress.

Please provide your kind suggestions.

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