How to increase the permeability of mouse Zona Pellucida?

More Mohamed Darwish's questions See All

What are the differences between pronuclear microinjection and cytoplasmic microinjection?

Generation of genetically modified mice using CRISPR/Cas9 system requires the delivery of CRISPR reagents into the genome. Some scientists use pronuclear microinjection of CRISPR reagents while...

11 December 2019 9,141 0 View

Is there any protocol explaining how to collect the mouse brain at the embryonic stages?

I want to collect the brain of mouse embryos at E11 and E18 stages for downstream qPCR experiments. Is there any protocol or video explaining how to extract the mouse brain at those embryonic...

11 December 2019 3,472 1 View

What is the best homogenizer to extract high-quality RNA for subsequent use in RT-PCR and RNA-sequencing?

We want to purchase a homogenizer that assure the extraction of a high-quality RNA; since RNA is fragile and easy to damage. Is there any recommendation?

06 July 2019 2,629 4 View

Could anyone kindly list for me the name of protocol publishing journals in the biology field?

I want to publish a new protocol in the biology field but I have a little knowledge about protocols and methods publishing journals. Could any researcher have experience about those kind of...

03 April 2018 2,070 8 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

What is the procedure to determine the permeability of concrete?

can anyone help me in this, it will help me with my research work

01 March 2021 834 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

Subhash C. Juneja

Curious, what is the experiment you are trying to do. You want in isolated zonae or live oocytes.

Mohamed Darwish

I want to do it in live oocytes

When one sperm enter the oocyte, there is natural process- block of ZP to polyspermy. If you try to increase permeability, the block to polyspermy may be jeopardized