Is there any method or protocol to increase the permeability of mouse Zona Pellucida to large molecules of around 100 nm diameter?
Curious, what is the experiment you are trying to do. You want in isolated zonae or live oocytes.
Subhash C. Juneja
I want to do it in live oocytes
When one sperm enter the oocyte, there is natural process- block of ZP to polyspermy. If you try to increase permeability, the block to polyspermy may be jeopardized
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