Mohamed Darwish

5 Questions 7 Answers 0 Followers

Questions related from Mohamed Darwish

Is there any protocol explaining how to collect the mouse brain at the embryonic stages?

I want to collect the brain of mouse embryos at E11 and E18 stages for downstream qPCR experiments. Is there any protocol or video explaining how to extract the mouse brain at those embryonic...

12 December 2019 3,508 1 View

How to increase the permeability of mouse Zona Pellucida?

Is there any method or protocol to increase the permeability of mouse Zona Pellucida to large molecules of around 100 nm diameter?

12 December 2019 488 3 View

What are the differences between pronuclear microinjection and cytoplasmic microinjection?

Generation of genetically modified mice using CRISPR/Cas9 system requires the delivery of CRISPR reagents into the genome. Some scientists use pronuclear microinjection of CRISPR reagents while...

12 December 2019 9,160 0 View

What is the best homogenizer to extract high-quality RNA for subsequent use in RT-PCR and RNA-sequencing?

We want to purchase a homogenizer that assure the extraction of a high-quality RNA; since RNA is fragile and easy to damage. Is there any recommendation?

07 July 2019 2,645 4 View

Could anyone kindly list for me the name of protocol publishing journals in the biology field?

I want to publish a new protocol in the biology field but I have a little knowledge about protocols and methods publishing journals. Could any researcher have experience about those kind of...

04 April 2018 2,100 8 View