How to increase the expression level of a yeast surface display peptide library (percentage of cells displaying the nanobody on its surface )?

I AM SCREENING A NANOBODY LIBRARY AND THE EXPRESSION IS UNDER GAL PROMOTER , THE INDUCTION IS DONE UNDER GAL PROMOTER AT 25 DEGREE C THE REPORTED EXPRESSION LEVEL IS 15% OF THE WHOLE POPULATION...

09 June 2025 1,065 0 View