Is there any integrated genome browser available to see chloroplast gene expressions?

Dear colleagues, Good Day! I am looking for any public database for visualizing RNA-seq data of chloroplast genes. For ex. I want to see the expression of Polycistronic mRNA abundancies of genes...

20 December 2020 7,474 3 View

Why it needs long time exposure to develop/get to see band images in western blot?

Hello Everyone, I'm checking expression for 4-HNE (abcam) (1:2000) by western blotting in mice skin tissue against UV stress. I'm facing struggle to get good visible bands, as I get band view only...

18 March 2020 5,800 3 View

Can we use DNA as template to do real-time PCR to check expression of gene?

Hello Everyone, I wanted to run RT-PCR using onestep real-time PCR systems and SYBR green (ThermoFischer) to check the gene expression in bacteria. I wanted to directly use 50 ng of DNA samples...

10 March 2020 1,965 3 View

Will the natural convection ( CFD simulation) problem within an enclosure converge thermally?.Because there is an external solar load as well?

I am solving the natural convection heat transfer problem within an enclosure with few heat generating components using Fluent.Around this enclosure,I have considered the fluid domain. To consider...

17 November 2016 6,131 5 View

How can I predict temperature intervals for REMD simulation to use with GROMACS ?

30 June 2015 1,205 2 View

How can I model the buckling of steel beams using contact modelling?

14 May 2015 6,256 5 View

How to extract DNA from Aspergillus spp. grown on solid medium?

Protocol for DNA extraction

11 July 2013 478 6 View

Why it is necessary to clone a plant gene in an entry vector before shuttling into an expression vector?

01 January 1970 5,005 4 View

Have you any idea about Trypsin digestion of proteins followed by SDS-PAGE. ?

Since, the trypsin digested peptides are very small in size, Is it possible to run them in SDS-PAGE and stain them (Silver stain).?Have anyone ever tried this method?

01 January 1970 5,114 9 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS?

I need to model masonry and reinforced concrete minarets for my MSc thesis. Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS? (Or any other program for just...

01 March 2021 736 3 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

How to find the right number of integration points in second order 3d finite elements?

I am familiar with finite element simulations and numerical schemes like the Gauss Legendre quadrature. I also know that the Gauss integration yields exact values for polynomials up to degree 2n-1...

27 February 2021 6,818 3 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View