How to inactivate bacteria without affecting the DNA?

More Nur thaqifah salihah hj mohd salleh's questions See All

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

What are the application of Fixed points thoery in Chemistry.?

It is said that fixed point theory has lot of applications not in the field of mathematics only but also in various areas. So I am looking for applications in Chemistry only..

26 February 2021 3,533 1 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Hye All, Greetings, How can i get surface run off water data?

this is for my thesis project, so this is the title of my thesis, the effect of land use on hydrological components in Muar, Johor, Malaysia any ideas, guidance, and suggestions all? Thank You, :)

02 February 2021 265 5 View

What is the molecular size for crystal violet and reactive orange 16?

I am using methyl violet 10B and reactive orange 16 (RO16) dyes for my Ph.D. research. I'm trying to find the molecular size for both of these dyes. It is possible for me to find these dyes'...

01 February 2021 3,333 4 View

How to find the target displacement to use in displacement control pushover analysis according to ASCE 41-17?

I am performing pushover analysis in Etabs of a steel braced frame but oI do not know what value should I use for target displacement. The height of the building is 20m. I want to know what value...

29 January 2021 2,190 2 View

Can we coat the pellet with graphite spray in place of silver paste for making electrical contact to measure the thermoelectric properties?

I want to coat the pellet because without electrical contact (may be due to the high resistance of the pellet), the V-I plot is not coming in the ZEM-3 instrument. I want to measure electrical...

24 January 2021 836 2 View

What is the difference of FTIR for functional properties and structural properties of protein?

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15 January 2021 8,992 1 View

What is suitable etching resist coating for polished Titanium Grade 5 surface?

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15 January 2021 1,592 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

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03 March 2021 3,568 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Which cell line should we use to perform biological dosimetry experiments?

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01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

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01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

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28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

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26 February 2021 4,777 4 View

Joaquim Ruiz

Dear Nur

If you want the DNA for PCR purposes it is not a problem to perform a heat inactivation. In fact is very frequent to extract bacterial DNA by boiling the bacteria at 100ºC for 10 minutes (or more), and (optionally) perform a short centrifuge step (only seconds), recover the supernatant. Thereafter this DNA may be used to perform PCRs.

Quim Ruiz

Mahmoud Ali Halablab

I suggest using sodium azide as it kills the bacteria at the same time it conserves the integrity.