I am working on a Gram negative bacterium and trying to isolate RNA for the NGS analysis. I have used trizol method as well as Qiagen kit based method. But the quality of the RNA is not good. I am attaching the RNA gel picture. This is the first time I am seeing something unusual like this. I have isolated RNA from cell line and other gram negative bacterium for which the quality was good. Please have a look at the gel and give your inputs to solve this problem and improve the quality.
Sourav Maiti
please note that RNA purity need to be checked. QAbsorbance at 230 and 260 nm wavelength is required. Usually Qiagen kits are excellent. You also need to monitor your procedure steps.
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