Hello,
I have some small secreted proteins which have the ability to move from plant to fungi. As they can move between plant and fungi, I assume these small secreted proteins have some domains responsible for their movement such as export and import domains. I am planning to generate a series of deletion constructs to identify domains that are responsible for protein movement. What might be the good experimental strategy to identify the domains? Is there any good system or experimental strategy that I can use for quick testing of my deletion constructs?
Thanks in advance
Hi Mahmud Hassan ,
Before I say anything, do you consider bioinformatic approaches to identify protein domains, or are you only looking for experimental methods?
Dear Bruno César Feltes Thanks for your reply. I used SignalP5.0 for predicting export domain. However, I did not find any software that can predict import domain (domain responsible for entry to the protein into the parasitic host). So, I am planning to make a series of C-terminal deletion construct and test their mobility (i.e entry into the target host). I am looking for a method that I can quickly test my deletion construct for import as well as export domain. Please let me know if you need more information.
- Badges
- Science topic
- Similar topics
- Chemistry
- General Biochemistry
- Proteomics