Mahmud Hassan

9 Questions 9 Answers 0 Followers

Questions related from Mahmud Hassan

How to identify domains in secreted protein?

Hello, I have some small secreted proteins which have the ability to move from plant to fungi. As they can move between plant and fungi, I assume these small secreted proteins have some domains...

05 November 2019 7,052 4 View

How to assemble four gblocks into a vector using Gibson assembly?

Hi All, I am trying to generate a bacterial operon construct. I have designed four gBlocks (each of which is ~1.7 kb) and synthesized them from IDT. I am using pGex-6p-1 vector to assemble these...

20 July 2019 4,291 3 View

Where to find pML-BART, pART7 and pART27 vector?

Hi All, I am looking for several plant expression vectors (pML-BART, pART27 and pART7). Could anyone please tell me where I can find these vectors? I have searched in google but did not get any...

08 July 2019 367 9 View

Does this abstract report any findings?

I would like to request researchgate people to comment on the following abstract. Does this report any findings, or convey a message? What are the weakness of this abstract? Improved design of...

10 December 2017 7,276 4 View

Could anyone explain why some Arabidopsis genes are present as single copy in Brassica napus genome?

I am trying to identify and elucidate the function of a gene (let say X) in Brassica napus that control flower development in Arabidopsis. Using the sequence of Arabidopsis X, I conducted a...

10 December 2017 7,783 2 View

Which is the best E. coli strain for unstable plasmid??

I am trying to make a plasmid by traditional cloning. The size of the insert is 7.3 kb and the size of the vector is about 7.2 kb. I am using PacI to isolate insert and prepare the vector. I had a...

21 July 2016 6,100 4 View

Where is the problem in my cloning?

I posted this problem before and Now I am posting it again as I am still having the same problem. Please see and help me to find the solution. "I am trying to clone a 7.3 kb insert into a plant...

19 July 2016 9,735 24 View

Which part of T-DNA enters the plant cell first?

T-DNA has two boarder sequence namely LB and RB. In case of Agrobacterium mediated transformation, which of this boarder area gets into the plant cell first? Also, which part are less prone to...

17 May 2016 7,252 2 View

How can I remove unwanted band from the positive clone?

I am trying to clone a 7.3 kb insert into a plant binary vector which is around 7.2 kb. The enzyme that I am using is PacI which has 2 bp overhang. I used electroporation and the cell that I used...

21 December 2015 4,304 6 View