I'm working with microsatellite (SSR) and get different allele sizes from the same primer.
The difference in alleles ranges from 1 - 4 bp. For example, in the first experiment I got alleles of 150 bp, 152 bp in the second, 150 bp in the third, and 154 bp in the fourth.
Is there any suitable software to analyze these different allele sizes so that a valid number is obtained which can be concluded from the different allele sizes?
Thank you
yes, This is the problem/error associated with gel-based genotyping, however, the error is reduced if we use automated genotyping machine and in one tube add from all the samples so that how many alleles are present at that locus u can know.
P.K.Meher,
thank you for your answer
I'm using a capillary electrophoresis machine and it seems the performance of each capillary matters.
Is it necessary to mix 1 DNA sample with a known fragment size in all samples to determine the accuracy of each capillary ?
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