I am getting the transition transfection of RSV in CHO S cells by doing Elisa. But after selection almost all the cells are dying. Please suggest what to do?
Even we tried less concentration of antibiotic and MTX for selection.
Could the RSV over-expression be cytotoxic to the cells?
Respiratory syncytial virus (RSV) infection of most cultured cell lines causes cell-cell fusion and death. Cell fusion is caused by the fusion (F) glycoprotein and is clearly cytopathic, but other aspects of RSV infection may also contribute to cytopathology.
https://jvi.asm.org/content/85/10/4792
Conference Paper An integrated cell line development platform for generation ...
An integrated cell line development platform for generation of high yielding CHO stable cell lines expressing a stabilized trimeric pre-fusion RSV F recombinant viral glycoprotein