I am trying reaction of peptides with small organic molecules in 30%DMSO Phosphate buffer (pH 7.0 ,0.1M). In all the LCMS spectra we do see intense solvent peak, due to which the starting material and product peaks are supressed, and I am finding difficult to get exact conversion on the bases of UV-profile.So please suggest me how to get rid of solvent peak from UV- profile.
Suggestions in no particular order:
Remove interfering solvent before injection (or use a more UV transp. solution).
Improve your method development to properly resolve all compounds apart (*This should always be the number one goal of all chromatography methods).
Use a switching valve to "Dump" the peak to waste after the injection so it does not contribute to the remaining results (See example device in attached link).
http://www.hplctools.com/lcmstruncate.htm
In general, I use 100% DMSO to overcome the solubility issues reported to some compounds. It is perfectly compatible with reverse phase (analytical and preparative scale). The co-elution must be chromatographically worked as previously suggested. (Try to use a good gradient elution). Regards.
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