Neelesh Reddy

10 Questions 7 Answers 0 Followers

Questions related from Neelesh Reddy

Is anyone tried to run Cytochrome C tryptic digest on UPLC?

We have ACQUITY UPLC Peptide BEH C18, 130Ao,1.7um, 2.1*150mm for Peptide mapping. The mobile phase which were tried 1) A: 0.045% H2O, B:.045%ACN 2)A: 0.045% H2O, B:.045%ACN Various gradient were...

18 June 2019 8,269 2 View

In column chromatography alumina can be used in place of silica as a stationary phase if the compound are sticking to silica ?

I have synthesized compound which contains amide bond, in column chromatography this compound sticking to silica? Is the use of alumina in place of silica resolve this problem?

16 January 2017 5,044 5 View

What parameters of MALDI -TOF one can control to get the the high resolution and better mass spectrum of high molecular weight protein?

UltraflextremeTOF from Brucker is the MALDI-TOF instrument and matrix Sinapinic acid (10 mg/ml), TA 50(50:50 ACN: H2O 0.1% TFA ) was used to record higher molecular weight protein like BSA, but...

13 January 2017 7,296 7 View

How to quench the t Bu cation and avoid the realkylation of cysteine with tBu in peptide cleavage ?

The peptide which was synthesized using solid phase peptide synthesizer have Trt protected cysteine and Boc-protected Lysine, we have used a cocktail 80:5::5:10 TFA: Phenol: Water: Tips.In this...

06 January 2017 7,714 3 View

Can I get the proteins which has single cysteine with molecular weight less than 30 kDa?having known PDB and good MALDI-TOF data?

Protein which has single cysteine with low molecular weight (Lower than 30 kDa), with Known PDB and good Mass data in MALDI-TOF. If the number of cysteines is more still its fine and useful.

06 January 2017 3,800 3 View

Does cysteine get alkylated with tButyl group in cleavage condition of peptides with Triisopropylsilane?

I am using Cleavage cocktail with 10% Tips, 5% phenol, 5% H2O, 80% TFA .I observed additional mass of 56 Da for the peptide I have synthesised.

03 January 2017 2,444 6 View

How to prepare a best sample for LCMS analyis?

If the peptides reaction were done at 30% DMSO and Phosphate buffer (0.1M, pH 7.0) with small organic molecule, so how to prepare the sample for this reaction mixture , If mobile phase for LCMS...

08 November 2016 9,019 1 View

How to get the peptide containing cysteine without oxidation?

I have synthesized few peptides containing cysteine, but in this case the peptide have sulfur in oxidized form (sulfoxide not disulfide).I am using solid phase peptide synthesizer.

03 August 2016 6,932 10 View

Can I get the reagent and condition for the activation of papain? and what are the different reageent which are used as inhibitors for papain?

Papain is the digestive enzyme which should activate before digestion.Certain reagents like cysteine, cyanide were already reported.

08 July 2016 8,184 2 View

Can I get PDB file of Betal-actoglobulin for two variants A and B?

Beta-lactoglobulin exist in two variants A and B (in this two variants the sequence of amino acid is different 64 position (AspA-GlyB) and 118 position (ValA- AlaB).

08 July 2016 9,584 1 View