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Questions related from Neelesh Reddy
We have Bruker Dalto ESI compass 1.3. Data analysis version 4.0 (build 234)
20 June 2019 2,170 0 View
We have ACQUITY UPLC Peptide BEH C18, 130Ao,1.7um, 2.1*150mm for Peptide mapping. The mobile phase which were tried 1) A: 0.045% H2O, B:.045%ACN 2)A: 0.045% H2O, B:.045%ACN Various gradient were...
18 June 2019 8,294 2 View
I have utilized Poroshell column 5μm 300SB C-18 (2.1*75mm). Solvent system Water: ACN. But I observed so many peaks in this case. Aldolase mol.wt. 39 kDa.
24 January 2017 7,345 3 View
I have synthesized compound which contains amide bond, in column chromatography this compound sticking to silica? Is the use of alumina in place of silica resolve this problem?
16 January 2017 5,062 5 View
UltraflextremeTOF from Brucker is the MALDI-TOF instrument and matrix Sinapinic acid (10 mg/ml), TA 50(50:50 ACN: H2O 0.1% TFA ) was used to record higher molecular weight protein like BSA, but...
13 January 2017 7,314 7 View
The native protein Aldolase A (mol. Wt. 39kDa) was showing the broadening of a peak in MALDI. Sometimes the spectra were too poor to analyze.
09 January 2017 6,585 3 View
The peptide which was synthesized using solid phase peptide synthesizer have Trt protected cysteine and Boc-protected Lysine, we have used a cocktail 80:5::5:10 TFA: Phenol: Water: Tips.In this...
06 January 2017 7,742 3 View
Protein which has single cysteine with low molecular weight (Lower than 30 kDa), with Known PDB and good Mass data in MALDI-TOF. If the number of cysteines is more still its fine and useful.
06 January 2017 3,820 3 View
I am using Cleavage cocktail with 10% Tips, 5% phenol, 5% H2O, 80% TFA .I observed additional mass of 56 Da for the peptide I have synthesised.
03 January 2017 2,466 6 View
If the peptides reaction were done at 30% DMSO and Phosphate buffer (0.1M, pH 7.0) with small organic molecule, so how to prepare the sample for this reaction mixture , If mobile phase for LCMS...
08 November 2016 9,038 1 View
I am trying reaction of peptides with small organic molecules in 30%DMSO Phosphate buffer (pH 7.0 ,0.1M). In all the LCMS spectra we do see intense solvent peak, due to which the starting material...
27 October 2016 4,304 2 View
I have synthesized few peptides containing cysteine, but in this case the peptide have sulfur in oxidized form (sulfoxide not disulfide).I am using solid phase peptide synthesizer.
03 August 2016 6,957 10 View
Papain is the digestive enzyme which should activate before digestion.Certain reagents like cysteine, cyanide were already reported.
08 July 2016 8,205 2 View
Beta-lactoglobulin exist in two variants A and B (in this two variants the sequence of amino acid is different 64 position (AspA-GlyB) and 118 position (ValA- AlaB).
08 July 2016 9,602 1 View
It's about the chemo selective reaction of amine or thiol
05 February 2015 1,268 3 View
